Susceptibility constants of escherichia coli and bacillus subtilis to silver and copper nanoparticles. Strain specificity in antimicrobial activity of silver and copper nanoparticles article in acta biomaterialia 43. Request pdf strain specificity in antimicrobial activity of silver and copper nanoparticles the antimicrobial properties of silver and copper nanoparticles. The mic and fractional inhibitory concentration index fici. The antimicrobial properties of silver and copper nanoparticles were investigated using escherichia coli four strains, bacillus subtilis and staphylococcus. Antioxidant effect of ag nanoparticles and silver nitrate in antimicrobial activity to confirm the effects of ag nanoparticles, a comparative study of ag nanoparticles and silver nitrate on antimicrobial activity against e. Shape dependent physical mutilation and lethal effects of. In this study, the antimicrobial activity of colloidal silver nanoparticles prepared by the solgel method was investigated, and the turbidity, viscosity and ph of the colloidal solutions were determined. Antibacterial agents are very important in the textile industry, water disinfection, medicine, and food packaging. Variation in sensitivity across various strains of bacteria and fungi. Ruparelia jp, chatterjee ak, duttagupta sp, mukherji s 2008 strain specificity in antimicrobial activity of silver and copper nanoparticles. Ruparelia jp, chatterjee a, duttagupta sp, mukherji s. Experimental antimicrobial effects of silver nanoparticles.
Silver, copper, gold, titanium, zinc have gained particular attention, each having different properties and spectra of action allaker, 2010. Antibacterial activities of gold and silver nanoparticles. The syntheses performed at various ammonia concentrations 0. Antimicrobial activity of metal oxide nanoparticles. Synthesis, electrochemical and antimicrobial activity of. Copper is well known for its antimicrobial activity and finds application in wound healing, skin remodulation and antiinflammatory therapies. Antibacterial activity of nanocomposites of copper and. Antimicrobial activity of silver and copper nanoparticles. In particular, silver nanoparticles agnps have attracted much attention in the scientific field 11. Strain specificity in antimicrobial activity of silver and. Strain specificity in antimicrobial activity of silver and copper. Antimicrobial activity of copper helps to reduce the microbial load at the site of wound and enhances the pace of healing.
Synthesis, characterization, and applications of copper. Yoon ky, hoon byeon j, park jh, hwang j 2007 susceptibility constants of escherichia coli and bacillus subtilis to silver and copper nanoparticles. Copperamino acids chelate was synthetized in aqueous solution and antimicrobial assays, which demonstrates that its strain specificities and enhanced activity are about ten times more effective comparing to the copper nanoparticles and the copperedta chelate, obtaining a better antimicrobial effect against e. Linoleic acid, absorption band, colloid, antimicrobial, microorganisms. Pdf strain specificity in antimicrobial activity of. Diseases such as tuberculosis tb have always had a large impact on human health. In this study, the antimicrobial activity of titanium dioxide tio2, zinc oxide zno, and tio2zno nanoparticles supported into 4a zeolite 4a z was. We compared the antibacterial effect of silver nanoparticles on gram negative and gram positive. Synergistic effects between silver nanoparticles and. Silver nanoparticles nanoags, which have wellknown antimicrobial properties, are used extensively in various medical and general applications. The antimicrobial properties of silver and copper nanoparticles were investigated using escherichia coli four strains, bacillus subtilis and staphylococcus aureus three strains. In this study, the combination effects between nanoags and the conventional antibiotics ampicillin, chloramphenicol and kanamycin against various pathogenic bacteria were investigated. Silver nanoparticles as potential antibacterial agents.
Characterization of copper nanoparticles synthesized by a. The minimum inhibitory concentration mic value of the. Antimicrobial potential of consolidation polymers loaded with. Synthesis of metal nanoparticles with antimicrobial properties has become a viable alternative and has promising applications in. Students research committee, department of food sciences and technology, faculty of nutrition and food. Antimicrobial properties of copper nanoparticles and amino acid. Characterization of copper nanoparticles synthesized by a novel microbiological method ratnika varshney, seema bhadauria,m. Metal agents such as silver, copper, zinc, titanium, and cobalt have antimicrobial properties. Antimicrobial activity and cytotoxicity against 4t1 mouse mammary tumor cell line. Ruparelia jp, chatterjee ak, duttagupta sp, mukherji s. In batch studies, a greater lag phase and lower maximum absorbance at 600 nm were observed as the concentration.
Characterization, and applications of copper nanoparticles, analytical letters, doi. Antibacterial mechanism of biogenic copper nanoparticles. Representative growth profile of microbial strains in the presence of varying concentration of silver and copper nanoparticles are depicted in fig. Silver together with copper, is commonly used to inhibit bacterial and fungal. In the study entitled strain specificity in antimicrobial activity of silver and copper nanoparticles, four strains of escherichia coli, three strains of each bacillus subtilis and staphylococcus aureus were used to assess the antimicrobial properties of silver and copper nanoparticles. The average sizes of the silver and copper nanoparticles were 3 nm and 9 nm, respectively, as determined through transmission electron microscopy.
Synthesis, electrochemical and antimicrobial activity of colloidal copper nanoparticles. The size of the silver nanoparticles was measured by atomic force microscopy afm. The brookhaven bi200sm goniometer was used in particle size measurement of suspended nanoparticles via. Synergistic antibacterial activity of a combination of silver and copper nanoparticle impregnated activated carbon for water disinfection. Antimicrobial properties of copper nanoparticles and amino. Copper nanoparticles as antibacterial agents omics international. A significant enhanced antimicrobial activity and a specific strain were. Biosynthesis, characterization and study of antimicrobial. Duttaguptab, suparna mukherjia, acentre for environmental science and engineering, indian institute of technology bombay, powai, mumbai 400 076, india b department of electrical engineering, indian institute of technology bombay, powai, mumbai 400 076, india. Surfaces of copper nanoparticles affect interact directly with the bacterial. The effect of charge at the surface of silver nanoparticles on antimicrobial activity against grampositive and gramnegative bacteria. The average sizes of the silver and copper nanoparticles were 3 nm and 9 nm, respectively, as.
Ruparelia jp1, chatterjee ak, duttagupta sp, mukherji s. Antifungal activity of silver and copper nanoparticles on two plant pathogens, alternaria alternata and botrytis cinerea. An experimentbased model quantifying antimicrobial activity of silver nanoparticles on escherichia coli mohammad a. Strain specificity in antimicrobial activity of silver and copper nanoparticles. Organic compounds used for disinfection have some disadvantages, including toxicity to the human body, therefore, the interest in inorganic disinfectants such as metal oxide nanoparticles nps is increasing. Nanoparticles nps have attracted great interest in drug development. Although there is some debate in the literature about the relative effect of nanoparticles on the type of bacteria, this study is in line with reports that suggest that gramnegative bacteria are more affected by. Silver nanoparticles and antibacterial activity nanoparticles are now considered a vi able alternative to antibiotics an d seem to have a high potential to solve the problem of the emergence of bacterial multidrug resistance 10. Alagar 2 1department of physics, pacr polytechnic college, rajapalayam626108, india. Abstract we discuss about the antibacterial activities of silver nanoparticles and compare them on both. A onestep simple synthesis of silver colloid nanoparticles with controllable sizes is presented. Antibacterial effects and their mechanism against pathogens volume.
Preparation and antibacterial activity of silver nanoparticles. Ruparelia j p, chatterjee a k, duttagupta s p and mukherji s 2008 strain specificity in antimicrobial activity of silver and copper nanoparticles acta biomater. The purpose of this study was to examine the potential of nps as antitb compounds by studying the interacting mechanisms between. Metals have been used for centuries as antimicrobial agents. Conversely, the antimicrobial tests revealed that the cu nanocomposites have antibacterial action for both bacteria, though with a more pronounced effect in respect to k.
More precisely, silver nanoparticles with size between 10 and 15 nm have increased stability, biocompatibility and enhanced antimicrobial activity yacaman et al. This paper reports a comparison of the antibacterial properties of copperamino acids chelates and copper nanoparticles against. Some studies have revealed that the antibacterial action of agnps is more effective against s. Ruparelia, jp, chatterjee, ak, duttagupta, sp, et al. The mic and mbc representing the antimicrobial activity of nanoparticles dispersed in batch cultures is summarized in table 1. Maryam azizilalabadi, mahmood alizadehsani, arezou khezerlou, mina mirzanajafizanjani, hajar zolfaghari, vahid bagheri, baharak divband and ali ehsani affiliation.
Antimicrobial potential of consolidation polymers loaded. Mechanistic basis of antimicrobial actions of silver. Ruparelia jp, duttagupta sp, chatterjee ak, mukherji s 2008a strain specificity in antimicrobial activity of silver and copper nanoparticles. Antimicrobial activity and prevention of bacterial biofilm. The antibacterial activities of biogenic copper nanoparticles were evaluated.
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